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Add diluent slowly: Draw 2.0 mL bacteriostatic water and let it run down the inside wall of the vial do not spray it directly onto the powder

In cohort 2, most patients were also female (68.1%), and the mean age was 50.6 (SD, 14.4)

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Avoiding Freeze-Thaw Cycles Repeated freezing and thawing of the reconstituted solution can cause aggregation or potency loss

Since D2HG and L2HG are indistinguishable in standard LC-MS, to determine which 2HG enantiomer(s) is affected, we adapted a chiral N-(p-toluenesulfonyl)-L-phenylalanyl chloride (TSPC) derivation followed by liquid chromatography with tandem mass spectrometry (LC-MS/MS) analysis 24,40

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