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Determination of nicotine, cotinine and trans-3-hydroxycotinine using LC/MS/MS in forensic samples of a nicotine fatal case by oral ingestion of e-cigarette liquid

During the high-temperature steam sterilization of the culture medium, an increase in temperature and pressure can enhance the ionization capacity of water, resulting in a greater release of H + and OH -

doi: 10.1038/nature22359

This is NOT NORMAL

After colony formation in each dish, the cells were washed with PBS several times and fixed by 4% polyformaldehyde for 30 min at room temperature
