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Western Blot Analysis HepG2 cells were seeded into a 6-well plate at 1 10 5 cells/mL and cultured for 24 h

154 A major obstacle in this process lies in the limitations of animal models, which remain indispensable for mechanistic studies and early phase drug screening

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Here, we addressed our mechanistic models experimentally using ScGrx7 as a reference enzyme 11,12,18

This knowledge coupled with the demonstration that some members of the 6-AH family bound with high affi nity to the hinge region of HGF led to the expectation that other active AnglV analogs, like 6-AH family members, could be expected to inhibit HGF dimerization and that the ability of an individual analog to bind HGF and inhibit HGF-dependent processes should be reflected in its capacity to attenuate dimerization

Further analysis revealed that CJC-1295 influence potentially led to an increase in total pituitary RNA and GH mRNA levels
