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[11] Qin ZH, Jin Y, Du YQ, et al

Protein was extracted from SH-SY5Y cells, N2A cells and mouse cortex using CellLytic buffer (Sigma) per manufacturer's instructions, separated by electrophoresis and blotted to PVDF membranes

Among the key regulatory protein which may be targeted by the dehydro-alanine glutathione analog are Keap1 Nrf2, the sensing cysteine thiolate of which can be chemically modified by electrophiles (such as the lipid-derived HNE) and triggers the transcription of genes for antioxidant proteins and for the intermediate metabolism [143]

Furthermore, evidence for changes in the level of blood or tissue mitochondrial function or metabolic readouts is currently lacking for these studies

Furthermore, studying local and systemic levels of SCFAs would be of great aid to understand the pathophysiological basis of MGB axis disruption in BD, especially focusing on its different phases and types