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Potential off-target effects can be suspected when the experimental results are opposite to what was expected but they are less likely pursued when the obtained results are consistent with the expectation

For this reason, researchers prepare acetic acid water fresh or work from single-use aliquots rather than multi-use vials

The association between APA and lung fibrosis is also supported by the link between the pro-fibrotic factor TGF1 and CFIm25 [320]

In some cells, however, selenium restriction reduces GPx-1 activity and protein to nearly undetectable levels with modest or no change in transcript levels (20, 56), suggesting that nonsense-mediated decay does not mediate suppression of GPx-1 protein expression under conditions of selenium restriction in all cells

Genomic Med

Medical University of South Carolina