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Rather than elute the protein with glutathione buffer, a PBS solution containing the enzyme is loaded onto the column and incubated for several hours at room temperature (4 C for PreScission protease)

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We also thank Alexei Rohou, Axel Brilot, Tamir Gonen, and Meghna Gupta for helpful discussions on cryo-EM map refinement and model building, and members of the Tomasiak lab and Robert Stroud for helpful discussions and critical reading of this manuscript

doi: 10.1038/s41467-020-15686-y 221 RyanJPKarimHTAizensteinHJHelblingNL

(2021), tanshinone IIA can inhibit LPS-induced chondrocyte inflammation and apoptosis by regulating the expression of miR-155 and FOXO3 signaling

Although macromolecular therapeutics offer several benefits, they encounter obstacles such as limited skin permeation and vulnerability to degradation