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The cells were cultured in DMEM/F-12 medium (11320033, Gibco, USA) supplemented with 5% horse serum and 2.5% fetal bovine serum at 37C under an atmosphere of 5% CO 2
The documentation states that the patient needed 1000mcg of B12 and the planned treatment was to administer 1 ml daily for 5 days
Just one hour sedentary will change the properties of the intervertebral disc in the lumbar spine
doi: 10.1097/00019442-200111000-00015
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