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Swelling of the mitochondrial outer membrane was performed by hypotonic shock, followed by sonication (four repeats of 5 s- bursts at high settings with 30 s pauses), supplementation with 150 mM NaCl and 5% glycerol and centrifugation at 20,000 g 30 min

In vitro, human ghrelin exhibits saturable transport mechanics in the blood-to-brain as well as brain-to-blood directions in a rat cerebral microvessel endothelial model [353]

doi: 10.1177/0148607113488799

ChenDQZhangHJZhangWFengKLiuHZhaoHLet al

Immunoaffinity media (detailed below) utilizes antigens' and antibodies' high specificity to separate

Nature 266 , 378380 (1977)