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IF analysis Following experimental treatments, cells were fixed with 4% paraformaldehyde (Sigma, F8775) for 15 min at 37 C

When exposed to zinc in vitro , DCs derived from bone marrow exhibited a tolerogenic profile, characterized by reduced surface MHC-II expression and increased levels of tolerogenic markers such as Programmed Death-Ligand (PD-L) 1, PD-L2 and the tryptophan degrading enzyme, Indoleamine 2,3-dioxygenase (IDO)
Zhang et al., 2017)