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Western blot analysis For protein analysis, cells or pieces of liver tissue were washed in PBS and lysed in Lysis Buffer (20 mM Tris-HCl pH8, 1 mM NaCl, 0.5% NP40, 0.5% Triton-X-100, 10% glycerol) supplemented with a protease inhibitor cocktail (SigmaAldrich)

PloS one 8, e81316

This leads to electron leakage from complexes I and III of the electron transport chain (ETC), resulting in the formation of superoxide radicals (O 2 ) [99]

[2] LOREN PICKART A M

Chemotherapeutic selectivity conferred by selenium: a role for p53-dependent DNA repair

To further investigate the potential relationship between MIA3 and CHAC1, we performed localization analysis, and both proteins were localized in the endoplasmic reticulum