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Chemical Composition of Cinnamomum verum Leaf and Flower Essential Oils and Analysis of Their Antibacterial, Insecticidal, and Larvicidal Properties. Molecules 26, no

coli (OD 600 0.5) were washed twice with sodium phosphate buffer (100 mM Na-phosphate buffer (Na 2 HPO 4 /NaH 2 PO 4 ), 0.5 mM EDTA, pH 7.5) and lysed using a triple freezing procedure (liquid nitrogen, 196C), defrosting (37C)

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Higher concentrations are achieved by using mixtures (for example 15% DMSO in combination with 15% ethylene glycol) which reduces the amount and toxicity of the individual cryoprotectants 10