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They are expressed in multiple tissues

Why the thigh is one of the best injection sites for tirzepatide Three sites are approved for subcutaneous tirzepatide injections: the abdomen, the front of the thigh, and the back of the upper arm

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Then, 0.5 ml chloroform and 0.25 ml H 2 O were added to complete the solvent system, and the lipids were extracted as described by Bligh and Dyer (31)

Whole blood was centrifuged at 3,000 rpm for 10 min to get plasma