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Understand its role in protein concentration determination, the significance of amino acids in UV absorption, and the application of molar absorptivity in concentration calculations.

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Purpose Paediatric obesity is a well-known risk factor for metabolic-associated fatty liver disease (MAFLD)

Parameters Sample 1 Sample 2 Sample 3 Expected Conc. (U/L) 12.6 32.5 65 Observed Conc. (U/L) 13.0 33.8 70.2 Recovery rate (%) 103 104 108

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