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The eluted protein was then buffer-exchanged with dialysis buffer (20 mM TrisHCl (pH 8.0) and 200 mM NaCl) and digested with TEV protease at 4 C overnight, to remove the Trx-His 6 -tag

On the other hand, STING1-mediated autophagy and MFN1 (mitofusin1)-MFN2-dependent mitochondrial fusion can increase ferroptosis sensitivity to various stimuli, including GPX4 inhibitors [186]

Ortiz-montero P, Londoo-vallejo A, Vernot J-P