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Cells were seeded into 12-well plates (1 10 5 cell/well), treated with 0.4 mM NAC, 0.4 mM NACET, or the vehicle for 16 h, washed with PBS three times and treated with 2 mM H 2 O 2 or 0.1 mM t-BOOH for 30 min before staining

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For instance, ossicle formation in Achilles tendon tissue appears with osteogenic protein 1 (OP-1) (Staresinic et al., 2003

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