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The persistent activity of striatal tyrosine hydroxylase indicates the preservation of dopaminergic neurons

As far as beneficial effects of NAC are concerned, the modulation of GLT-1 seems to be crucial for diminishing the excitatory glutamatergic transmission observed after drugs of abuse

Previous studies in seabream indicate that 24 h of feed deprivation was too short to induce metabolic changes, whereas 48 h were sufficient to induce a hyperphagic response (54, 55), and in 7 days, long-term energy stores were almost completely depleted (56)

TLR4 activation triggers various pro-inflammatory processes in microglia, including an augmented production of ROS [88]

doi: 10.1038/s41467-021-27493-0 36 Hernandez-MontelongoJ.LucchesiE

Hence, in the current study the direct effect of NAC on endothelial cells can't be ruled out and needs further investigation