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Membranes were blocked in TBS-T containing 5% skim milk for 1 h at room temperature and then incubated with primary antibodies against -catenin, phosphorylated ERK (p-ERK), and -actin (all from Santa Cruz Biotechnology, USA)

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Unveiling the role of melatonin MT2 receptors in sleep, anxiety and other neuropsychiatric diseases: a novel target in psychopharmacology