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Description
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The intraday of the NAC method was checked by injecting six individual preparations of standard (0.005 mg/ml) and placebo sample (0.005 mg/ml) within the calibration range
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The slides were incubated with fluorescently labeled secondary antibodies (Alexa Fluor 488-conjugated goat anti-mice immunoglobulin G, Beyotime, China or Alexa Fluor 488-conjugated goat anti-mice immunoglobulin G, Beyotime, China) for 2 h at 37C and 4,6-diamidino-2-phenylindole (DAPI) (#c-1002, Beijing, China) for half an hour at room temperature, and finally, the slides were sealed with antifluorescent quencher and nail polish
