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Heat shock protein 47 (HSP47) is a collagen-specific molecular chaperone that is indispensable for the proper folding and secretion of collagen into the extracellular space

10.1021/es303228n 69 ShimH

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Depending on the target material, this can include the use of detergent or other buffers, proteinases or other enzymes, heating to various times/temperatures, or mechanical disruption such as cutting with a knife or homogenizer, using a mortar and pestle, or bead-beating with a bead mill

For cadmium, the cacao plant absorbs the metals from the soil and levels accumulate in the beans