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4T1 cells (mutant or non-mutant) constructed above were transfected with lentivirus for 72 h and then screened with puromycin at a concentration of 1.5 g/ ml to construct a cell line stably expressing luciferase

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Clinical signs of B12 deficiency include nausea, vomiting, loss of appetite, poor absorption of food (malabsorption syndrome), constipation, gas, weight loss, fatigue, lethargy, abdominal pain, and diarrhea
V.YamamotoM.et al (2018)

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