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Total RNA extraction Approximately 50,000 synchronized adult worms were collected with M9 using a glass Pasteur pipette

CryoGrid preparation LPL at a concentration of 1 mg/mL or higher was mixed with 1 mM sodium deoxycholate and dialyzed into 500 mM NaCl, 20 mM Tris-HCl pH 8 at 4 C either with or without 1 mM deoxycholate in the dialysis buffer

Transient or weak interactions may be difficult to detect with AP-MS, depending on the stringency of the washing steps prior to elution of bound proteins

The concomitant changes in the levels of PC and PE have significant implications

It is often attractive because 3S packs may already be available, easier to reuse across models, or easier to fit into certain layouts

An increase in the expression of mRNA related to the translation of SVCT2 in both neurons and astrocytes has been proven in rats with induced cerebral ischemia, probably as a cellular protective mechanism against oxidative stress induced by ischemia (Berger et al., 1995) and gluthation (Puskas et al., 2000) as well as the possible therapeutic applications of this interaction, e.g., in the prevention of Alzheimer's disease (Morris et al., 1998)