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The tissues were homogenized using a high-speed drill on ice in a glass Dounce homogenizer in Mitochondria Isolation Buffer (containing in mM 210 sucrose, 2 EGTA, 40 NaCl, 30 HEPES, pH 7.4)
BMC Pregnancy Childbirth 5 , 15 (2005)
MIC B12 is metabolic support
Using hydrogen peroxide-induced senescent TM3 Leydig cells as an in vitro model, we observed that FOXO4 maintains the viability of senescent Leydig cells and suppresses their apoptosis
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