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Then siRNA-Akt was used to determine that L-carnitine ameliorated cancer cachexia via the Akt/FOXO3/MaFbx

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Osteosarcoma (OS) is the most prevalent and aggressive primary malignant bone tumor, characterized by early metastasis and a poor prognosis, particularly in patients resistant to conventional multimodal therapies

Cell viability was evaluated by MTT assay, apoptosis by flow cytometry using annexin-V/propidium iodide staining, and protein expression of apoptosis-related markers (Bax, Bcl-2, and caspase-8) by western blot

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