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Tissues were permeabilized with PBS supplemented with 0.1% Tween-20 for 10 min, followed by a peroxidase block with 3% H 2 O 2 (Sigma-Aldrich, 216763) for 20 min

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Thus, we additionally screened for the presence of N-terminal extensions to determine the presence or absence of a putative targeting peptide (Additional files 2, 3, 4, 5, 6, 7, 8)