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In a study that was conducted to evaluate the role of oxidative stress in melasma, the levels of glutathione peroxidase enzyme activity and other pro-oxidant parameters were significantly higher in the blood of patients compared to controls

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doi: 10.1016/j.mce.2016.09.028, [DOI] [PubMed] [Google Scholar] Karagiannis A., Gallopin T., Lacroix A., Plaisier F., Piquet J., Geoffroy H., et al

Briefly, the crude enzyme extract was prepared by combining the composite mango pulp sample (from 8 fruit per replication) at eating soft ripe stage (1 g) with a reaction mixture (10 mL) including potassium phosphate buffer (100 mmol L 1 , pH 7.0), polyvinyl pyrrolidone (PVP, 2.5%), EDTA (1 mmol L 1 ) and L-ascorbate (1 mmol L 1 ), and homogenised and centrifuged (25 min at 15,000 g , 4 C)

Caldwell J

Acta 1762 , 256265 (2006)
