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The tissues were blotted on filter paper, weighed, and homogenized in 0.05 M phosphate buffer (pH7.5) using a tissue homogenizer at 40C
(5) A control group should be included for comparison with the ALD group
The homogenate was spun at 18,000 g for 15 min and the supernatant was used for subsequent analysis
Indeed, conditional and epithelial-specific knockout of IGF-1 results in reduced mammary branching during ductal growth [53]
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doi: 10.1007/s12975-016-0509-z 162 LiuYZhengYKaratasHWangXFoerchCLoEHet al
