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This is primarily due to platelet activation during the blood collection process and is a known issue of concern with some eicosanoids (e.g., TxB 2 , 12-HETE)

V.S., N.M., A.F

Zinc and reproduction: Effects of zinc deficiency on prenatal and early postnatal development

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288 Furthermore, dysregulation of Wnt3a, -catenin, and HIF-1, as well as antioxidant enzyme activities (Mn-SOD, Cu/Zn-SOD, glutathione and CAT), synergistically promotes oxidative stress by inhibiting the Wnt/-catenin and HIF-1 signaling pathways in the liver during I/R injury