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1d, Supplementary Figs 1c and 5)
Barth PG
Eur J Pharmacol 641:2934
The role of free radicals and antioxidants in reproduction
Skin fibroblasts were cultured and the activity of propionyl CoA carboxylase was determined in vitro by incubation with propionate, malonate and [14C] sodium bicarbonate, followed by acidification to release carbon dioxide from unmetabolised bicarbonate, and measurement of the radioactivity remaining in the incubation medium.
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