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Within the RNAi mechanism in plants, the process is initiated by siRNAs that are typically 21 24-nt long

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The 4th generation used site-saturation mutagenesis with degenerate NNK codons (or MNN at the reverse primer) to (i) introduce an extra linker residue at the C-terminus of linker 2 or (ii) to randomize the amino acid composition of Ngo2014 K174-H176 region

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This means it is made of three specific amino acids: cysteine, glycine, and glutamine