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doi: 10.1016/j.eururo.2023.04.003 130 KimHShimBYLeeSJLeeJYLeeHJKimIH

We thank C.-W

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To further elucidate the evolutionary relationships of BxGST3 and BxGST1 , a phylogenetic tree was constructed using the maximum likelihood (ML) method in MEGA12 software based on the amino acid sequences of these two genes and GSTs from other representative species ( Figure 2B )

Electrophoresis was performed for 3 h at 150 V at room temperature using the MOPS (1 M Tris, 2% SDS, 20 mM EDTA
