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Despite the long history of AILI research, intracellular processes, in particular how hepatocytes ultimately die, are still not fully understood and controversial

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The deletion of the flbA gene can promote the cell death and autolysis of A

Methods Cell culture Human lung adenocarcinoma cell lines (A549, H322, H1299, H1792, H1975, H2009, PC9) were previously obtained 87 from the Harmon Cancer Center Collection (University of Texas-Southwestern Medical Center) or commercially from the American Type Culture Collection (ATCC) and cultured in RPMI 1640 medium (Fisher Scientific, 11-875-119) supplemented with 5% FBS (Sigma-Aldrich, F0926) in the absence of antibiotics