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After 48 h incubation, cells were seeded at low density to establish clonal cell lines following brief selection with 1 g/ml puromycin for 23 days

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Among these mechanisms, the loss or functional impairment of GABAergic inhibitory interneurons is recognized as one of the core mechanisms underlying hyperexcitability within epileptic networks

Part of the reason for this differential effect is that the metabolite of glutamine, -kG, alters gene expression programs that support an anti-inflammatory M2-like state

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