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doi: 10.1093/cid/ciab147 39 ElkattawySAlyacoubRAyadSPandyaMEckmanA

Protein ubiquitination forms an isopeptide bond between the C-terminal carboxyl group of ubiquitin and the -amino group of lysine residues on target proteins

(Reference source: Proof of concept: Pull down assay using bovine serum albumin and an immunomodulator small molecule. The type of Pull-down experiment The type of Pull-down experiment According to the purpose and design of the experiment, Pull-down experiment can be divided into the following types : GST Pull-down GST-tagged bait protein binds to glutathione agarose beads

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Mobile phase A (0.1% formic acid in water) and B (0.1% formic acid in acetonitrile) were used for gradient elusion at 0.4 mL/min at 45 C

However, the mean values of accessory sex organs of NaF+CE group were significantly higher than those of NaF treated group (Table 1)
