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The connections between ferroptosis and bone and joint diseases involve mainly iron metabolism, ROS, GPX4, and lipid peroxidation processes

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One possible explanation for these apparently conflicting results is that SOD2 protein levels are upregulated through post-translational mechanisms, such as reduced activity of the ubiquitinating enzyme USP36 48 , and that subsequently a feedback adaptation reduces mRNA levels (e.g., via miRNA)

sub-millimolar range

The lipofectamine 2000 (Invitrogen, MA, U.S.) reagent was used to transfect cells with the appropriate amount of vector

We monitored glutamate release to culture medium and confirmed that erastin acts via system X c inhibition (Fig