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This class of RNA includes a wide variety of non-coding regulatory RNAs as well as messenger RNAs mRNAs, which transfer genetic information from DNA to the translational machinery for protein synthesis

Total RNA was isolated from cells using TRIzol reagent (Invitrogen)

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Clarke R, Peden JF, Hopewell JC, Kyriakou T, Goel A, Heath SC, Parish S, Barlera S, Franzosi MG, Rust S, Bennett D, Silveira A, Malarstig A, Green FR, Lathrop M, Gigante B, Leander K, de Faire U, Seedorf U, Hamsten A, Collins R, Watkins H, Farrall M