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ROS analysis Cells were incubated with 5 M CM-H 2 DCFDA for ROS, or 5 M C11-BODIPY (581/591) for lipid ROS in the dark for 30 min at 37 C

Therefore it is essential to investigate the effect of GSH depletion on RPE cells to further elucidate the potentially involved mechanisms

For long-term storage, freezing at -20C helps keep it pure and stable

Beyond direct intracellular delivery, NAC also operates through a fascinating mechanism known as "thiol exchange." In the bloodstream, much of our natural cysteine is bound up in an oxidized, unusable form called cystine

Nolan, G