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It is used exclusively in controlled laboratory environments for scientific exploration and not approved for human use

[PubMed] [Google Scholar] Robert A, Nezamis JE, Lancaster C, Davis JP, Field SO, Hanchar AJ

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However, it has become clear that the most widely used enzyme-linked immunosorbent assays (ELISA) do not detect zonulin but complement C3 and possibly properdin, a related molecule from the same family with unclear functional effects (73, 74)