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To obtain the Retromer trimer, the GST-tagged Vps29 and Vps35 co-expressed cell pellet was mixed with His-Vps26A and resuspended in lysis buffer containing 50 mM Tris-HCl pH 7.5, 200 mM NaCl, 2 mM 2-Mercaptoethanol, 50 g/ml benzamidine and DNase I before being processed through a Constant System TS-Series cell disruptor
[PMID: 17602868]
doi: 10.1016/0005-2744(76)90098-x
Figure 4 3.2.1.3 Asthenozoospermia Sperm nuclear DNA damage is one of the important mechanisms leading to asthenozoospermia
Cario H, Bode H, Debatin KM, Opladen T, Schwarz K
Toxicity can occur in therapeutic use or after overdose
