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Uchida A

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Exp Neurobiol 25 , 233240 (2016)

Hellstrom, W

3) For large volume of supernatant, concentrate the proteins by ammonium sulphate precipitation, dialyze the dissolved protein solution against 1 PBS, and then apply the solution onto the GSH column

Moreover, the hydrazone bond formed between the cyanine hydrazide and protein carbonyls is usually sensitive to acidic environment and narrow pH range conditions are required to optimize the efficient binding of cyanine-hydrazide fluorescent dye to its carbonyl targets