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Cell damage and integrity during permeabilization has been assessed by fluorescent probe uptake assays (such as 1-phenylnaphthylamine or NPN) [78], protein cell leakage, transmission electron microscopy (cell structural alterations) and transport assays with L [ N-methyl 14 C]carnitine (specific carrier transport studies) [76]

At the end of the treatment period, after euthanizing the animals, serum levels of LH, FSH, testosterone, insulin and glucose were analyzed, and histological, immuno-histochemical (IHC), and molecular examinations of the ovaries were performed

Epigenetic regulation, such as DNA methylation and non-coding RNAs, has also been identified to modulate key proteins in both ferroptosis and cuproptosis, offering new perspectives for co-targeting (81, 82)

Ludovici V, Barthelmes J, Ngele MP, Enseleit F, Ferri C, Flammer AJ, Ruschitzka F, Sudano I