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After repeated washing the bound fraction was eluted with 250 mM imidazole (Fischer) in lysis buffer containing 10% glycerol (Acros)

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in this way, it plays a key role in the development of myocardial dysfunction ( Fine particulate matter (PM2.5) can produce a large number of ROS and reactive nitrogen species (RNS), caused the unbalance of cell homeostasis and then excessive ROS may result in damage of nuclear DNA and mitochondria including mitochondrial permeabilization, membrane potential decreasing and mitochondrial swelling, followed by further apoptosis in cardiomyocytes (Yang et al., 2018)

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[DOI] [PubMed] [Google Scholar] 121.Lopes da Silva S., Vellas B., Elemans S., Luchsinger J., Kamphuis P., Yaffe K., Sijben J., Groenendijk M., Stijnen T