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Description
zinc sulphate 30% was used for deproteinization of the samples (0.5 ml of supernatant of brain homogenate + 0.5 ml of ZnSO 4 )

(41) Additionally, it stimulates glucose uptake by cells, particularly in muscle and adipose tissues, by activating the insulin pathway and increasing the expression of glucose transporters such as GLUT4

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doi: 10.1002/anie.202315286

Office of Dietary Supplements, National Institutes of Health

The supernatant was discarded and samples were resuspended in 5 Laemmli buffer
