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To terminate the cultures, 20 L of 0.1% Triton X-100 and 30 L of lyticase (1 mg/mL in 0.1 M phosphate buffer, pH 7.5, containing 50 mM mercaptoethanol) were added, and the plates were incubated for 1 h at 35C

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The peripheral venous hematocrit and hemoglobin concentration did not reflect the hypovolemic state and the significant reductions in the red blood cell volume and plasma volume