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Cells were first stained with 50 M DCFDA or 10 M mBcl, as described above, followed by a washing step with PBS and a surface staining of 30 min on ice with combinations of the following monoclonal antibodies: PE-conjugated anti-CD10 (HI10), PerCP-conjugated anti-CD3 (SK7), APC-conjugated anti-CD19 (SJ25C1), PE-conjugated anti-CD5 (L17F12), PE-conjugated anti-CD25 (PC61), all from BD Biosciences
A fundamental limitation of both rodent and human oligodendroglia monocultures is their inability to form compact, multilamellar myelin in the absence of axons, confining their utility largely to differentiation rather than functional myelination studies
Corresponding author: Edwin Lee, MD Email: [email protected] INTRODUCTION Interstitial cystitis Moderate to severe interstitial cystitis is a disabling chronic bladder disorder also known as bladder pain syndrome
Previous study indicated that in response to mounting Cu, MTF1 binds to metal-responsive elements (MREs) in the promoter of PRNP and activates its transcription 23
Esteban-Figuerola et al
