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To measure the effect of the paramagnetic label, 1 H, 15 N and 1 H, 13 C HSQC spectra with long inter-scan delays (3 s) of 100 M 13 C/ 15 N-labeled p53 TAD2 in presence of one stoichiometric equivalent paramagnetically labeled FOXO4 FH were recorded before and after addition of 5 mM ascorbic acid

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Dosing Protocol The murine-equivalent dosing framework reproduced from published preclinical studies (not a human protocol)

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mouse monoclonal anti-GFAP antibody, cell signaling technology, #3670, 1:250) for double labeling