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The cells were lysed by sonication in lysis buffer (20 mM TrisHCl, pH 7.5, 500 mM NaCl, 1 mM EDTA, 0.5% TritonX100, protease inhibitor cocktail from Roche) and centrifuged at 12,000 g for 15 min

Gastroenterology and Hepatology from Bed To Bench , 15
doi: 10.1016/j.tips.2024.05.006

Several of these metabolites have already been explored as candidate biomarkers and therapeutic targets in preclinical studies [165]

Garca-Matas S, Paul RK, MolinaMartnez P, Palacios H, Gutierrez VM, Corpas R et al (2015) In vitro caloric restriction induces protective genes and functional rejuvenation in senescent SAMP 8 astrocytes
Recent epidemiological studies have identified the glucose 6-phosphate dehydrogenase (G6PD) gene on the X chromosome as one possible location of such genetic variants
