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Moreover, this approach is unable to differentiate individual components of the glycocalyx, as the majority of stains used to visualize the glycocalyx are cationic molecules such as lanthanum, Alcian blue and ruthenium red, thus their binding properties are contingent upon charge and not a specific glycan (204)

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Moreover, the inclusion of only RCTs led to some studies being of lower quality due to a lack of blinding and insufficient outcome data