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Forjaz MJ Article: JOURNAL OF CLINICAL MEDICINE

Fox, S.W

Key factors in homologous recombination (HR), including breast cancer type 1/2 susceptibility protein (BRCA1/2) and RAD51, as well as components of the Fanconi anemia (FA) pathway, are essential for faithful DSB repair [36,37,38]

has shares in and is CEO of Sphingotec GmbH, the company having patent rights on the sphingotest bio-ADM assay

The diagnostic approach: Run time course: Remove samples at 1h, 2h, 4h, overnight Analyze by SDS-PAGE Look for: Extra bands (degradation products), decreasing band intensity (target destruction) The solutions: Failure 3: Protease Inhibition (Your Buffer Kills the Enzyme) The problem: Proteases have specific requirements and sensitivities: Common mistakes: IMAC elution buffer: High imidazole inhibits thrombin Reducing agents: DTT reduces thrombin activity Metal chelators: EDTA kills metalloproteases Wrong pH: All proteases have pH optima The solution: Always buffer exchange into protease-compatible conditions before cleavage

Chemotherapy and radiation therapy can damage intestinal cells, leading to glutamine deficiency