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Samples were reconstituted in 100 L and 2 L of the sample was injected from a 4 C autosampler into a ZIC-pHILIC 150 2.1 mm 5 m particle size column (EMD Millipore) with a ZIC-pHILIC 20 x 2.1 guard column in a Vanquish Duo UHPLC System (Thermo Fisher Scientific) at 25 C

In agreement with these findings, Abd-Elrazek and Ahmed-Farid found that L-carnitine significantly decreased busulfan-induced elevated oxidative stress markers (MDA, glutathione disulfide (GSSG), nitric oxide (NO), and 8-hydroxyguanosine (8-HdG)) and significantly increased busulfan-induced glutathione (GSH) with antioxidant activity (53)

Since studies using conditional knockout mice indicate that muscle, but not hepatic, IGF1 is a critical factor for skeletal growth (Bikle et al., 2015), it is possible that L-carnitine activates the mTOR signaling by increasing local IGF1 production

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