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The rats were euthanized, and tissue samples (brain, heart, kidneys, liver, spleen, lung, stomach, intestine, muscle, grease, ovaries, womb, testicles, and thymus) were collected at 3 min, 10 min, 1 h, and 24 h after administration (three males and three females at each time point)

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This is typically performed under local anaesthesia

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